Tangential Flow Filtration (TFF) Optimization

Tangential Flow Filtration (TFF) is used extensively in biopharmaceutical industries for clarification of microbial cells/animal cells, inclusion bodies clarification, protein concentration and diafiltration, buffer exchange, viral clearance, etc. Multiple Centrifugation steps can be avoided by a well designed TFF system with an optimized protocol.

TFF Process Development
The first step in the development of a TFF Continue reading Tangential Flow Filtration (TFF) Optimization

Physical methods of Cell Disruption – Homogenizer

Cell disruption is needed for the extraction of intracellular products. The method used may vary depending on the type of cell and its cell wall composition. Irrespective of the method used, the main aim is that the disruption must be effective and the method should not be too harsh so that the product recovered remains in its active form.

There are different Continue reading Physical methods of Cell Disruption – Homogenizer

Filtration in BioProcess – Tangential Flow Filtration – TFF

Filtration is the process of separating the components of a solution based on their size and charge differences using a filter. It is a pressure driven process. Filtration is an important process in biopharmaceutical industry which is used right from the separation of cell mass to the final concentration of the product.

Types of Filtration
Based on the fluid flow, filtration can be Continue reading Filtration in BioProcess – Tangential Flow Filtration – TFF

The CIP Procedure

In continuation to an earlier description about what a CIP system is, the CIP procedure follows

The CIP Procedure:
CIP (Cleaning In Place) is usually accomplished through the action of chemicals by spraying or pressure re-circulating the flushing, washing, and rinsing solutions under the controlled conditions of time, temperature and chemical concentration. The flushing solution Continue reading The CIP Procedure

CHEMICALS / SOLUTIONS USED DURING CIP

As discussed in the previous topics, the CIP or Cleaning in Place is today, a mandatory requirement in any process industry especially in pharmaceutical and biopharmaceutical. The case in Pharma and BioPharma is very severe because of contamination issues and regulatory conformance. CIP must be validated to ensure proper cleaning.

Some of the mandatory requirement of CIP solutions is Continue reading CHEMICALS / SOLUTIONS USED DURING CIP