Guidelines for Installation of Shaft Mechanical Seal

The following list provides a guideline for successful installation of Mechanical seals. This is a result of the project work done by Mr C. Vijayakumar for his Diploma in Mechanical Engineering at IGNOU.

1. Always handle mechanical seals with extreme care. Cleanliness is imperative. Never place faces face down on bench or floor. Continue reading Guidelines for Installation of Shaft Mechanical Seal

Tangential Flow Filtration (TFF) Optimization

Tangential Flow Filtration (TFF) is used extensively in biopharmaceutical industries for clarification of microbial cells/animal cells, inclusion bodies clarification, protein concentration and diafiltration, buffer exchange, viral clearance, etc. Multiple Centrifugation steps can be avoided by a well designed TFF system with an optimized protocol.

TFF Process Development
The first step in the development of a TFF Continue reading Tangential Flow Filtration (TFF) Optimization

Physical methods of Cell Disruption – Homogenizer

Cell disruption is needed for the extraction of intracellular products. The method used may vary depending on the type of cell and its cell wall composition. Irrespective of the method used, the main aim is that the disruption must be effective and the method should not be too harsh so that the product recovered remains in its active form.

There are different Continue reading Physical methods of Cell Disruption – Homogenizer